Journal: Journal of breast cancer

Article Title: Norepinephrine/β 2 -Adrenergic Receptor Pathway Promotes the Cell Proliferation and Nerve Growth Factor Production in Triple-Negative Breast Cancer.

doi: 10.4048/jbc.2023.26.e25

Figure Lengend Snippet: Figure 5. Norepinephrine/β2-adrenergic receptor signal promotes the growth of triple-negative breast cancer cells via ERK activation. (A) Phosphorylation of ERK and CREB after NE treatment of MD-MB231 cells. After starvation, the cells were treated with 10 µM NE/ISO/Ate/Pro/ICI for 6 hours. Cell lysates were subjected to immunoblotting with antibodies against p-ERK (1:1,000), ERK (1:1,000), p-CREB (1:800), CREB (1:800), and β-actin (1:1,000). Quantitation of the western blotting results is shown in the bar graph. Data from three independent experiments are expressed as means ± SD. (B) Phosphorylation of ERK and CREB after NE treatment of BT549 cells. Data from three independent experiments are expressed as means ± SD. (C) CCK-8 assay of the MD-MB231 and BT549 cells. After starvation, MD-MB231 and BT549 cells were treated with 10 µM NE/ISO/KT5720/U0126 for 24 hours to measure the cell viability. Data are shown as means ± SD from triplicate experiments. NE = norepinephrine; ISO = isoprenaline; Ate = atenolol; Pro = propranolol; SD = standard deviation; CCK = Cell Counting Kit; β2-AR = β2-adrenergic receptor. *p < 0.01, †p < 0.001.

Article Snippet: Anti-tyrosine hydroxylase (TH) Ab (75875), anti-S100 Ab (112), and anti-β2-AR Ab (182136) were purchased from Sigma (St. Louis, USA); anti-CREB Ab (12208-1-AP), phosphorylated anti-CREB Ab (ser133), anti-ERK Ab (16443-1-AP), Phosphorylated anti-ERK Ab (T202/Y204), and anti-NGF Ab (52918) were purchased from Cell Signaling Technology (Danvers, USA); anti-beta-actin Ab was purchased from Beijing Zhongshan Jinqiao Company (Beijing, China); anti-TrkA BAS-bs (10210R) was purchased from Beijing Biosynthesis Biotechnology Co., Ltd. (Beijing, China); horseradish peroxidase (HRP)-conjugated Goat Anti-Rabbit IgG (H+L) (SA00001-2) was purchased from Proteintech Group (Rosemont, USA).

Techniques: Activation Assay, Phospho-proteomics, Western Blot, Quantitation Assay, CCK-8 Assay, Standard Deviation, Cell Counting

Journal: Oncology Letters

Article Title: Increased soluble E‑cadherin of spheroid formation supplemented with fetal bovine serum in colorectal cancer cells

doi: 10.3892/ol.2023.13793

Figure Lengend Snippet: Densitometric results of western blotting on SNU-C5 and SNU-C5/5-FUR cells.

Article Snippet: CREB (1:1,000; cat. no. CSB-PA005947HA01HU; Cusabio Technology, LLC), fibronectin (1:2,000; cat. no. CL54951AP; Cedarlane Laboratories), p21 (1:1,000; cat. no. 60214-1; Proteintech Group, Inc.) and p90RSK (Ab348; 1:1,000; cat. no. 79-554; Prosci, Inc.) were obtained from the corresponding listed company.

Techniques: Western Blot, Marker